Welcome and thanks for visiting the Keats Lab website,
This site was developed with the goal of providing the international community a portal into the results of many of the projects ongoing in the lab. We plan to share many of our protocols, resources, and knowledge about the biology of multiple myeloma, the genetics of multiple myeloma, and the available model systems.
The Keats Lab is part of the Integrated Cancer Genomics Division at the Translational Genomics Research Institute (TGen) in Phoenix, Arizona. Our primary research interest is a blood cancer called multiple myeloma. This cancer is a relatively rare cancer diagnosed in approximately 20,000 people each year in the United States, which translates into an incidence of around 5.7/100,000 individuals. Unfortunately, this cancer is quite aggressive resulting in 3.6/100,000 deaths each year. Our primary focus is to understand the genetics underlying this cancer and what genetic events occur between diagnosis and the development of a tumor, which is refractory to therapy. The lab also has secondary interests in other blood cancers and immunodeficiency syndromes.
Summer 2014 - Moving Day
Dec 7, 2014
Ash Poster can be downloaded from this link. (ASH2014_Clonal_Modeling)
May 27, 2014 - POST-DOCs WANTED
We are currently recruiting motivated post-docs with an interest in myeloma biology and genomics. Please forward a cover letter and CV to Dr. Keats if you are interested. Interested candidates will be directly involved in the analysis of data generated from the MMRF CoMMpass project. Candidates with backgrounds in biology and/or bioinformatics are invited to apply.
April 11, 2014 - SEARCHABLE DATABASE RELEASED
We have created a searchable website for the cell line characterization dataset. You can query by gene or by cell line to see which assumed somatic events have been detected. (https://myelomagenomics.tgen.org/)
December 12, 2013
Our poster presented at ASH is now available for download.
Comprehensive Genomic Characterization of All Commercially and Non-Commercially Available Multiple Myeloma Cell Lines
Nov 14, 2013 - DATA RELEASE UPDATE
Updated copy number tables are now available for the entire cell line series. This includes a raw data matrix and segmentation files.
April 17, 2013 - DATA RELEASE
Today we released the first preliminary full dataset of the cell line characterization project. You can access the preliminary mutation calls from the exome sequencing, and expression estimates from the mRNA sequencing on the Data Repository Page
April 10, 2013
Our poster on the genomic characterization of an isogenic UTX model system at AACR is available for download.
April 8, 2013
Check out the ecancer.tv interview of Dr. Keats after his presentation at the International Myeloma Workshop in Kyoto Japan.
April 4, 2013
The slides from the presentation Dr. Keats gave at the 14th International Myeloma Workshop are now available for download.
December 9, 2012
Our posters being presented at ASH are now available for download.
November 20, 2012
September 18, 2012
We hit a major milestone today with the cell line characterization project today as we have officially completed all tissue culture associated with the project on the entire cohort of 65 cell lines. In the next month we will finish the RNAseq, Exome sequencing, and CGH on the remaining 32 cell lines.
August 10, 2012
Today was the last official day for David who oversaw most of the cell line characterization project. In recognition of his hard work we are releasing the first segmentation file representing the CGH findings from the 33 cell lines completed to date. This file is design for the IGV browser available from the Broad Institute. Along with David's departure for graduate school we also had to say a very sad good-bye to Kim last week when she left for graduate school and sadly Amy will also be returning to school next week. Best of luck with your studies we will miss having all three of you around.
June 26, 2012
In just under a year we have already had more than 1000 unique visitors to the site from 54 countries and more than 50% of visitors have returned more than once.
April 23, 2012
The cell line characterization project is expanding to include 4 additional cell lines that have become commercially available after the initial cell line orders were completed (PCM-6, JIM3, Karpas25, and Karpas929). Plus an additional panel of cell lines were kindly provided by Leif Bergsagel and Diane Jelinek bringing the total cell line count to 65.
March 18, 2012
Exome sequencing results for all 32 cell lines in the characterization project are complete and analyzed. Exome captured by Agilent SureSelect Human All Exon V4+UTRs. Data will be available by request next week and publicly available in the very near future.
Feb 17, 2012
All cell lines in the characterization project were tested on Agilent 400k CGH arrays this week. Data will be available by request next week and publicly available in the very near future.
Jan 18, 2012
Tissue culture for all 32 cell lines being analyzed in the Myeloma Cell Line Characterization Project is now complete. All genomic characterization should be completed by Feb/March depending in sequencer availability.
Jan 9, 2012
Preliminary run report and non-synonymous variant table for KMS11_JPN is available for download and viewing on the data repository page
Nov 15, 2011:
We are happy to report that over 100 unique visitors have visited the website to date. (Stats: 134 visitors from 26 countries have visited the site 309 times)
Nov 1, 2011:
TruSeq exome enrichment and sequencing for UAMS isogenic cell line series ARD, ARP, CAG and the adherent KMS-11 sub-line called KMS11_JPN is complete.